In the present study, we performed quantitative trait locus qtl analysis for rsv resistance using 98 backcross inbred lines. Genetic mapping of quantitative trait loci qtls to identify genomic regions controlling grain yield under drought and subsequent utilization of markers linked to the qtls in markerassisted pyramiding of desirable alleles is an effective alternative to breeding high yielding drought resistant rice. For past decades, many researchers have identified qtl for peduncle vascular bundle related traits in rice. Frontiers qtl identification for cooking and eating quality. Pdf qtl mapping for crude protein and protein fraction. Qtl mapping of panicle blast resistance in japonica.
However, the recent severe conditions of flooding because of climate change, which has. Nine loci responsible for seed vigor were identified via qtl mapping by using a highdensity bin map. Quantitative trait loci mapping for bacterial blight resistance in rice using bulked segregant analysis. Rice, panicle blast resistance, qtl, fine mappin g, mas, introgression lines, resistance breeding background rice oryza sativa l. The development of megavarieties with tolerance conferred by the sub1 gene on. To identify quantitative trait loci qtl controlling heat tolerance in rice, the progeny of bc1f1 and f2 populations derived from an ir64 n22 cross were exposed to 3824 c for 14 days at the. In the present study, we performed quantitative trait locus qtl analysis for rsv. Genetic mapping and validation of quantitative trait loci qtl for the grain appearance and quality traits in rice oryza sativa l. The production of rice, however, is faced with many challenges like the problem on salinity. Quantitative trait loci qtl analysis for rice grain width. Qtl analysis for rice grain length and fine mapping of an identified. Pdf rice is one of the most important cereals that feed more than half of the worlds population. Submergence is a common naturally occurring disaster in rice production in south and southeast asia. Doerge in the past lo years, interest in applying the tools of molecular genetics to the problem of increasing world rice production has resulted in the generation of two highly saturate molecular linkage maps of rice, and the.
Simulation study showed that qtl seq is able to detect qtls. Qtl mapping of low temperature on germination rate of rice. These results also confirmed that qtl mapping via association mapping using a magic population is a powerful method in genetic analysis of complex traits. A total of 4 ect qtls corresponding to 4 hd qtls were mapped. Oct 16, 2018 thus, we speculate one major qtl exists in the region of pl9. Pdf high resolution mapping of qtls for heat tolerance. Pdf genetic mapping and validation of quantitative trait.
A major qtl controlling deep rooting on rice chromosome 4. However, the quantitative trait loci qtls and genes related to panicleblast resistance have not been well studied due to the timeconsuming screening methodology involved and variation in symptoms. Asian cultivated rice, was most frequently used in yield. Qtl mapping is an efficient strategy to dissect the molecular mechanisms of rice yield traits such as ngp 4. Qtlseq to rice recombinant inbred lines and f 2 populations and successfully identi. Qtl mapping of low temperature on germination rate of rice 95 0 5 10 15 20 25 30 5 15253545 5565758595 germination rate % no. In this study, four advanced backcross populations were selected for qtl mapping of panicle components in rice. Qtl mapping works very well to find single genes with large effects.
Genetic base on peduncle vascular bundle related traits in rice was systematically dissected, and most likely candidate genes of the known gene nal1 and the three newly identified qtl qlvn6, qsvn7, and qsva8. Incidence of heat stress at reproductive phase of the crop results in abnormal pollination. Assessing trait contribution and mapping novel qtl for. A quantitative trait locus qtl is a locus section of dna that correlates with variation of a quantitative trait in the phenotype of a population of organisms. A qtl is shown as an interval across a chromosome where the probability of association is plotted for each marker used in the mapping experiment. Mapping of novel qtl regulating grain shattering using doubled haploid population in rice oryza sativa l. Approaches to qtl mapping traditional qtl mapping utilizes biparental population bp mapping populations from controlled crosses zhu et al. Pdf importance of qtl mapping for heading date in rice. Mapping qtls of root morphological traits at different.
If repeatability qtl phenotyping experiment low qtl map very unreliable. Qtls are mapped by identifying which molecular markers such as snps or aflps correlate with an observed trait. Qtl mapping for crude protein and protein fraction contents in rice oryza sativa l. Pdf wx is considered to be the most important gene controlling eating and cooking qualities and pasting properties in rice oryza sativa l. Zhang j, chen k, pang yl, naveed sa, zhao xq, wang xq, wang y, dingkuhn m, pasuquin j, li zk, xu jl 2017 qtl mapping and candidate gene analysis of ferrous iron and zinc toxicity tolerance at seedling stage in rice.
Locations of markers goals identify the or at least one genomic region, called quantitative trait locus qtl, that contributes to variation in the trait. Primary studies have identified qtls associated with disease resistance, abiotic stress tolerance and yield potential of rice in a range of ecosystems. Qtl mapping of grain quality traits in rice sciencedirect. Qtl mapping and gwas reveal candidate genes controlling. Many qtls for traits related to salinity tolerance have been mapped. Development of nutritious rice with high zincselenium and. Qtl analysis and dissection of panicle components in rice.
Single plant analysis of each of the polymorphic marker was carried out using re 2. Natural variation in growth and physiology under salt stress in rice. Substitution mapping of qtl for rice quality and phenotype ac and rva. Mapping quantitative trait loci qtl associated with cooking quality in rice oryza sativa l. Mapping and introgression of qtl for yield and related traits in two. Fine mapping of stable qtls related to eating quality in rice oryza. To identify new qtls for root growth angle in rice, we selected three accessions with shallow rooting from different varietal groups of cultivated rice, and produced f 2 mapping. The asian pool is dominated by indica varieties and includes diverse kernel size and shape market classes, while u. Qtl will be useful for rainfed rice improvement, and will also contribute to our understanding of the genetic.
Here, the indica rice sea rice 86 sr86 seedlings showed higher tolerance than ordinary rice varieties in saline soil, and a dominant effect. Qtl analysis for rice salinity tolerance and fine mapping of a candidate locus qsl7 for shoot length under salt stress article pdf available in plant growth regulation december 2019 with 65 reads. Overall these novel findings would improve the knowledge of temperature on rice flowering. Qtl mapping for thermosensitive heading date in rice. Rice is one of the most important cereals that feed more than half of the worlds population. Doerge in the past lo years, interest in applying the tools of molecular genetics to the problem of increasing world rice production has resulted in the generation of two highly saturate molecular linkage maps of rice, and the ioculization of numerous genes and quantitative trait loci qtls. Qtl mapping of chlorophyll contents in rice sciencedirect. Fine mapping of a panicle blast resistance gene pbbd1 in. Therefore, developing salttolerant and highyielding rice using quantitative trait loci qtls and linkage markers is a priority for molecular breeding. Qtl mapping of physiological traits at the booting stage in rice under low temperature combined with nitrogen fertilization. Frontiers identification of a novel qtl for panicle length. Simulation study showed that qtlseq is able to detect qtls over wide ranges of experimental variables, and the.
Heat stress is one of the major abiotic threats to rice. Mapping qtls for submergence tolerance in rice using a population fixed for sub1a tolerant allele. Kanbar and others published mapping qtl associated with root and related traits in dh population of rice find, read and cite all the research you need on researchgate. Identification of qtls for yield and agronomic traits in rice under. Chromosome 8 of rice is shown as a vertical open bar with mapped markers indicated to the left ref. Grain quality improvement is one of the most important goals in a rice breeding program. Inheritance and qtl mapping of salt tolerance in rice 27 lod2. Although the history of genetic studies about heading date is very long, however marvelous break through in this field has started after the discovery of qtl mapping technique n i 1980. The donor parent was an indica superhybrid rice restorer line, hr1128, which was previously chosen to be a highyield qtl donor for high throughput genetic analysis. A total of 19 single nucleotide polymorphisms snps were found to be significantly associated with seed vigor. This is an impossibly large window in which to look for a gene without some clear idea of what it might be. Mapping of novel qtl regulating grain shattering using. Pdf qtl analysis of agronomic traits in rice using ssr and. While at least 546 qtl were detected in primary mapping.
Quantitative trait loci qtl underlying related traits at the seedling stage under two different phosphorus levels was investigated in rice using a population of 76 chromosomal sequence. Genotypingbysequencing based qtl mapping for rice grain. Timerelated mapping of quantitative trait loci controlling. The rice blast caused by magnaporthe oryzae is one of the most devastating diseases worldwide, and the panicle blast could result in more loss of yield in rice production. Association mapping of yield and its components in rice. Quantitative trait loci qtl analysis for rice grain width and fine mapping of an identi. Qtl mapping for salinity tolerance in rice oryza sativa using ultrahigh density snp. Moreover, studies on qtl mapping of rice tolerance against low temperature at. Frontiers genetic mapping of quantitative trait loci for. Qtl mapping of chlorophyll contents in rice 21 detected in interval rg978rm210b on chromosome 8. Saline stress severely affects rice oryza sativa l.
Fine mapping of qstv11kas, a major qtl for rice stripe. Atefeh sabouri1,2, babak rabiei1, mahmoud toorchi2, saeed. Association mapping of yield and its components in rice cultivars. The number of qtl was detected in nonsaline 14 and saline soil 10. Pdf the main objective of this study was to dissect the genetic basis of rice flowering regulated by temperature. Qtls mapping was then performed based on the 17 linkage markers and the f 2 segregating population, and finally three qtls. Qtl mapping of grain quality traits using introgression lines carrying oryza rufipogon chromosome segments in japonica rice yeotae yun1, chongtae chung1, youngju lee1, hanjung na1, jae. Pdf qtl mapping for salinity tolerance in rice oryza. Rice stripe disease, caused by rice stripe virus rsv, is one of the most serious diseases in temperate rice growing areas. An indica variety with small grain size was crossed to a japonica variety with large grain size to construct a set of recombinant inbred lines rils which was used to identify quantitative trait loci qtls controlling eight grain quality traits. Relationship between coleoptile length and drought resistance and their qtl mapping in rice 15 shorter than male parent irat109 in cl table 1. Interval mapping and composite interval mapping identified four qtl qna1. Mapping quantitative trait loci qtl associated with cooking quality.
Jul 10, 2018 these qtls are directly relevant and useful in breeding for cooking and eating qualityceq in indica rice. The major ect qtl, qehd10, was repeatedly identified on chromosome 10, which could explain 42. Qtl mapping with an example css population in rice consisting of 65 nonidealized css lines and 82 chromosome segments indicated that a total of 18 segments on eight of the 12 rice chromosomes harboured qtls a. Jan 19, 2016 submergence is a common naturally occurring disaster in rice production in south and southeast asia. Emphasis was given to qtl colocation and phenotypic. Identification and validation a major qtl from sea rice 86. Pdf mapping qtl associated with root and related traits. Qtl mapping for thermosensitive heading date in rice article pdf available in euphytica 2051 february 2015 with 91 reads how we measure reads. Qtl mapping and candidate gene analysis of peduncle. Further the most likely candidate genes for each important qtl were also discussed based on functional annotation. Qtl mapping of combining ability and heterosis of agronomic traits in rice backcross recombinant inbred lines and hybrid crosses.
In the past 10 years, interest in applying the tools of molecular genetics to the problem of increasing world rice. Primary studies have identified qtls associated with disease resistance, abiotic stress tolerance and yield. Quantitative trait loci mapping for bacterial blight. High resolution mapping of qtls for heat tolerance in rice using a 5k snp array shanmugavadivel ps1,2, amitha mithra sv1,chandraprakash1,ramkumar mk1, ratan tiwari3, trilochan mohapatra4 and nagendra kumar singh1 abstract background. Qtl mapping in rice pdf map position, qtgw5 seemed to be the same gene as qsw5, which controls. Qtl seq to rice recombinant inbred lines and f 2 populations and successfully identi.
Pdf qtl analysis for rice salinity tolerance and fine. Pdf qtl mapping for thermosensitive heading date in rice. Qhd10, without highresolution mapping, and the reality of qtls identified by interval mapping. Qtl regions can also be quite large, incorporating too many genes to investigate as potential candidate genes. Importance of qtl mapping for heading date in rice. Qtl mapping requires a phenotypic screening system with high h. Jun 27, 2005 in this study, an attempt was made to clarify the genetic basis of the grainfilling mechanism in rice using timerelated qtl mapping wu et al. These beneficial qtls can be used as targets for molecular breeding and can be further investigated to.
Therefore, it is possible to generate rice lines with high znse and low cd by a well. Pdf mapping quantitative trait loci qtl associated. Traditional qtl mapping is highly dependent on the genetic diversity of the two parents, and the effects of the detected qtls can vary between populations. The gramene qtl database includes qtl identified for numerous agronomic traits in rice, maize, barley, oat, sorghum, pearl millet, foxtail millet and wild rice. Qtl databases have become a routine tool for complex traits analysis in functional genomic research and qtl mapping is widely used due to its simplicity and concept 1. Dissection and finemapping of two qtl for grain size linked. Dissection and finemapping of two qtl for grain size. Inheritance and qtl mapping of salt tolerance in rice.
Qtl mapping for milling quality in elite western u. Quantitative trait locus qtl mapping 10810, cmb lecture 9 eric xing backcross experiment. May 30, 2019 a total of 19 single nucleotide polymorphisms snps were found to be significantly associated with seed vigor. Mapping mim are also extensively used in mapping of complex traits. Shu ming yang, fei fei zhang, su hua zhang, gui yong li, li qiong zeng, guan. For grain size and weight, qtl cloned are small in number and include no minoreffect qtl. We performed qtl mapping for physiological traits related to rice salttolerance. In this study, the genetic basis of grain length was dissected into six maineffect quantitative trait loci. High resolution mapping of qtls for heat tolerance in rice. Qtl mapping of physiological traits at the booting stage.
The qtls for panicle blast resistance have been mapped in a population of 162 rils recombination inbreeding lines, derived from a cross between a highly blastresistant rice landrace. In this study, a genetic analysis of quantitative trait loci qtls. Original article open access dissection and finemapping of two qtl for grain size linked in a 460kb region on chromosome 1 of rice qing dong1,2, zhenhua zhang1, linlin wang1, yujun zhu1,2, yeyang fan1, tongmin mou2. Shb resistance were identified on rice chromosomes 2, 3, 5, 7, 9, and 11 using segregating f2 populations. Based on data from an affymetrix rice genechip, two genes related to starch. Heat stress is one of the major abiotic threats to rice production, next to drought and salinity stress. Qtl nomenclature was followed according to mccouch et al 15. Genetic variation and association mapping for 12 agronomic. Qtl mapping of grain length in rice oryza sativa l.
Natural variation in growth and physiology under salt. Qtl mapping of combining ability and heterosis of agronomic. The parameters such as additive effects and variance explained were also estimated. With a mapping population constrained by the difficulties of phenotyping it may be 2030 cm. Pdf mapping qtl for heat tolerance at flowering stage in.
An indica variety with small grain size was crossed to a japonica variety with large grain size to construct a set of recombinant inbred lines rils which was used to identify quantitative trait loci qtls. Quantitative trait loci qtl analysis for rice grain. Genotypingbysequencing based qtl mapping for rice grain yield under reproductive stage drought stress tolerance. Because some of these major qtls have large intervals, fine mapping, candidate gene identification and development of qtl. In the past 10 years, interest in applying the tools of molecular genetics to the problem of increasing world rice production has resulted in the generation of two highly saturated, molecular linkage maps of rice, and the localization of numerous genes and quantitative trait loci qtls. The use of qtl mapping has contributed to a better understanding of the genetic basis of many agronomic important traits. Qtl mapping very inaccurate for detecting, localizing, and estimating the effect size of genes with a small effect. The identification and mapping of qtls for such traits has great significance for rice breeders. Integrating gwas, qtl, mapping and rnaseq to identify.
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